Abstract,
Good control, efficiency, and reproducibility of protein extraction from cells and tissues are essential aspects for diverse biological and basic research applications. Effective and specific enzymatic digestion of proteins prior to mass spectrometry （MS） analysis is one of the fundamental techniques most commonly used in any proteomics laboratory. However, neither routine procedures for cell and tissue lysis nor for in-solution and in-gel protein digestion have been significantly altered in common practice for over a decade. Here we have tested and optimized several alternative techniques for preparing lysates of mammalian cells, as well as in-solution and in-gel enzymatic digestion compatible with downstream qualitative and quantitative MS-Based proteomics applications.

摘要，
從細胞和組織提取蛋白質的技術中, 良好的控制，效率和可重復性對于不同生物的分析和基礎研究的應用是一個非常重要的觀念。在質譜（ MS ）分析前, 有效和具體的蛋白酶解是一種蛋白質組學實驗室中zui常用的基本技術。然而，無論是細胞和組織溶解的例行程序，或是凝膠蛋白質消解, 這十多年已被大大改進。基于蛋白質組學應用, 我們進行了幾個哺乳動物細胞裂解的測試和優(yōu)化, 以及在質譜（ MS ）的基礎下進行in-solution and in-gel蛋白酶解的定性和定量分析。
 

    本轉載文章均出于公益目的，僅供網友學習與交流，并僅提供參考，不作為任何依據。本所載文章內容涉及著作權等問題，敬請權利人與我們，我們將及時處理所轉載的文章。

     有關詳情請瀏覽培安公司的www.pynnco.com,電子郵件：sales@pynnco.com, ：。


 

SPP 超高壓循環(huán)樣品制備系統(tǒng)